The Agencourt CleanSEQ system is a rapid, high performance dye-terminator removal process based on the Agencourt SPRI® technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performed manually or fully automated for high throughput dye-terminator removal. The Agencourt CleanSEQ system produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.


Application: Cycle sequencing clean up

Downstream Application Techniques: DNA Sequencing

Agencourt CleanSEQ Features:
  • Long Phred 20 read lengths
  • Increased fluorescent signal intensities
  • Easily automated
  • Compatible with most common dye-terminators and sequencing instrumentation
Agencourt CleanSEQ Benefits:
  • Produces higher quality sequencing data
  • Reduces sequencing costs
  • Enables increased BigDye1 dilution
  • Saves time


1. Add Agencourt CleanSEQ reagent and ethanol to sequencing reaction 2. Bind sequencing products to magnetic beads 3. Separate sequencing products from contaminants with magnetic field 4. Wash with ethanol 5. Elute from magnetic particles 6. Transfer away from magnetic beads

How Agencourt SPRI Technology Saves Money
The Agencourt SPRI technology can significantly reduce sequencing costs. A typical sequencing lab annual budget before and after using Agencourt CleanSEQ is shown below. The overall operating cost can be substantially reduced by using the Agencourt CleanSEQ system. The pie chart on the left shows 200,000 reactions per year, with a dye-terminator volume of 4 µL/reaction and a reagent cost of $0.60/reaction resulting in a $554,040 annual budget cost. The pie chart on the right shows the same number of reactions with the same labor cost. However, by incorporating the Agencourt CleanSEQ reagent and a Beckman Coulter Biomek FX Automated Workstation, the resulting package reduces the annual operating budget to $412,993, which is a savings of $141,047.*


Superior Sequencing Data
The Agencourt CleanSEQ system produces high sequencing pass rates and average Phred 20 read lengths greater than 650 base pairs. Results from direct comparison against EtOH precipitation, gel filtration and silica-based magnetic reagents demonstrate the Agencourt CleanSEQ kit's superior performance. The process consistently delivers higher signal-to-noise ratios and overall signal intensities, longer Phred 20 read lengths, and is more reproducible than alternative clean up methods. This enables rapid, more cost effective sequencing.


Average Signal Strength

Identical 96-well plates were sequenced using BigDye v3.1 Terminators, dye terminators removed using either Agencourt CleanSEQ or ethanol precipitation and sequences delineated on an ABI 3730xl.
Consistent Performance

Several 96-well plates were sequenced using BigDye v3.1 Terminators and read on an ABI 3730xl. Dye terminators removed using either Agencourt CleanSEQ or DyeEx1 kit following manufacturing instructions.


Scalable and Automation-Compatible
The Agencourt CleanSEQ protocol does not require precipitation, filtration or centrifugation. The Agencourt SPRI technology is easily scaled and automation friendly, allowing both high throughput and format flexibility. Agencourt Bioscience has written and optimized software methods for Beckman Coulter's Biomek® Laboratory Automation Workstations.


Assumptions: Labor is assumed constant at 35% of overall reagent cost. This will vary from lab to lab and may decrease due to the automation component of the technology. In this example, dye-terminator cost is $4.50/reaction.
1. All trademarks are property of their respective owners.

Available for use in your laboratory
To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.


Product Size Kit Components Product #
Agencourt CleanSEQ Kit (8 ml) 800/1,600 preps* Agencourt CleanSEQ Reagent 000121
Agencourt CleanSEQ Kit (50 ml) 5,000/10,000 preps* Agencourt CleanSEQ Reagent 000136
spacer
Related Products Product #
Agencourt SPRIPlate® 96R - Ring Magnet Plate 000219
Agencourt SPRIPlate® 384 Magnet Plate 000222
Agencourt SPRI 384-Well Direct Inject Plate 000296
Agencourt SPRI 96-Well Direct Inject Plate 000596
*96 or 384 well format

Please contact your sales representative or distributor with any questions. In the U.S. and Canada, call us at 800-361-7780. Outside the U.S. and Canada, call us at 978-867-2600. Email us at webinfo@agencourt.com.