Agencourt Bioscience, a Beckman Coulter Company, is pleased to announce the launch of the Agencourt AMPure XP kit, a robust post-PCR cleanup solution, which outperforms traditional post-PCR cleanup methods.

The Agencourt AMPure XP system offers enhanced features over the current Agencourt AMPure chemistry including:

• Processing time 40% faster than the original Agencourt AMPure kit
• Consistent recovery throughout the extended shelf life of 12 months
• Efficient recovery of both double stranded and single stranded DNA templates

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Used by many top genomic centers, the Agencourt AMPure system is a highly efficient, easily automated PCR¹ purification system that delivers high quality DNA. Requiring no centrifugation or filtration, Agencourt AMPure purifies both small and large PCR extension products in 96- or 384-well formats.

Application: PCR purification

Downstream Application Techniques: Genotyping applications (SNP, fragment analysis), clinical sequencing, sequencing, resequencing

• Efficient removal of unincorporated dNTPs, primers and salts
• High-quality amplicon purification
• No centrifugation or filtration
• Manual or fully automated formats
• Clean up of a variety of enzymatic reactions including PCR, rolling circle amplification and cDNA labeling

The Agencourt AMPure system removes unincorporated primers, dNTPs, DNA polymerases and salts used during PCR amplification that can interfere with downstream applications. Agencourt AMPure routinely produces recovery rates greater than 90% for amplicons larger than 200bp and greater than 85% for smaller PCR amplicons, such as the 165bp fragment shown in Figures 1 & 2. Samples purified using Agencourt AMPure are ideal sequencing templates and routinely produce Phred 20 values of greater than 650 bases.

Figure 1: Shown are amplified pGEM2 plasmid fragments of 165, 321, 410, 508 and 967 bp. Half of the reactions were purified using the Agencourt AMPure kit, and the percent recoveries calculated using a PicoGreen2 assay. Samples were run on a 4% agarose gel. Lane MW: 100bp ladder; Lanes 1, 3, 5, 7 and 9: unpurified PCR products; Lanes 2, 4, 6, 8 and 10: Agencourt AMPure-purified samples.

Figure 2: Fragments sizes of 165, 321, 410, 508 and 967bp from the M13 phage were amplified using PCR, purified using the Agencourt AMPure kit and the percent recovery calculated.

The Agencourt AMPure protocol does not require precipitation, filtration or centrifugation. The Agencourt SPRI technology is easily scaled and automation friendly, allowing both high throughput and format flexibility. Agencourt Bioscience has written and optimized software methods for Beckman Coulter's Biomek® Laboratory Automation Workstations.

To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.

¹ The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.
² All trademarks are property of their respective owners.