The Agencourt Chloropure system rapidly isolates both DNA and RNA from plant material. The use of Agencourt's patented Solid Phase Reversible Immobilization (SPRI®) paramagnetic bead-based technology makes this extraction kit highly amenable to automation and has been designed for use with Beckman Coulter Biomek Laboratory Automation Workstations.

Application: DNA and RNA extraction from plant material

Downstream Application Techniques: PCR¹, qPCR, qRT-PCR, sequencing, genotyping, microarray

Kit Features:

  • Significantly higher recovery of nucleic acid over other commercially available methods
  • High 260/280 ratio
  • Perfect for downstream PCR applications
  • Processes up to 3 plates in approximately 80 minutes using a Biomek Workstation
  • Automation friendly with no vacuum filtration needed


1. Homogenization of plant material 2. Binding of nucleic acids to magnetic beads and separation 3. Washing of captured nucleic acids with wash buffer 4. Washing with 70% ethanol to remove contaminants 5. Elution of nucleic acids from the magnetic particles

Excellent Recovery from Many Plant Species
The Agencourt Chloropure system is versatile enough to extract nucleic acid from different plant types. As seen in Figure 1, the Agencourt Chloropure kit recovers significantly more nucleic acid from cotton, sunflower, maize, and canola samples than the MagAttract² 96 DNA Plant kit.

Figure 1. DNA yield from 18 replicates of cotton leaf punches, sunflower leaf punches,maize leaf punches and canola seeds purified using the Agencourt Chloropure kit or MagAttract 96 DNA Plant kit and analyzed using a Nanodrop² spectrophotometer.

High Purity
Contaminants can negatively affect downstream applications. As seen in Figures 2, 3, and 4, nucleic acid concentrations for the Wizard² Magnetic 96 DNA Plant system cannot be accurately determined with spectrophotometric analysis due to an extremely high A230 peak from residual buffer salts that masks the A260 peak, resulting in an inflated measurement.

Figure 2. A) Spectrophotometric analysis of a maize leaf punch purification using the Wizard Magnetic 96 DNA Plant system. B) Spectrophotometric analysis of a maize leaf punch purification using the Agencourt Chloropure system.

Summary
The Agencourt Chloropure purification system is simple, efficient, and automation friendly when used with Beckman Coulter laboratory automation and Agencourt software methods. By using the power of SPRI chemistry, the Agencourt Chloropure system produces more DNA and RNA over competitive methods without salt contamination or PCR inhibition.

Available for use in your laboratory
To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.


Product Size Kit Components Product #
Agencourt Chloropure Kit - Small 384 preps
(4 x 96)		 Lysis Buffer
Wash Buffer
Bind Buffer		 A47949
Agencourt Chloropure Kit - Large 9,600 preps
(10 x 96)		 Lysis Buffer
Wash Buffer
Bind Buffer		 A47951
spacer
Related Products Product #
Software - Agencourt Chloropure 96 MC - v 3.x A47948
Software - Agencourt Chloropure 96 MC - PS/FX - v 3.x A47947
Please contact your sales representative or distributor with any questions. In the U.S. and Canada, call us at 800-361-7780. Outside the U.S. and Canada, call us at 978-867-2600. Email us at webinfo@agencourt.com.


¹ The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.
² All trademarks are property of their respective owners.