The Agencourt CosMCPrep system is an Agencourt SPRI® paramagenetic bead-based plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated without sacrificing product quality. The single protocol, ease of automation and high quality results make Agencourt CosMCPrep an ideal solution for all your plasmid purification needs.

Application: High and low copy plasmid purification

Downstream Application Techniques: DNA Sequencing

Agencourt CosMCPrep Kit Features:
High quality plasmid purification Superior downstream sequencing results
Automated protocols Single protocol for all template types


1. Resuspension of pelleted cells in RE1 solution and lysis of bacterial cultures with L2 solution. 2. Addition of N3 solution. Flocculent formed in lysate. 3. Transfer of clear lysate from under flocculent to a fresh 96-well round bottom microtiter plate. 4. Addition of isopropanol and PUR4 solution to bind DNA of interest to magnetic beads. 5. Separation of DNA bound to magnetic beads from contaminants. 6. Washing of DNA with Ethanol. 7. Elution of purified plasmids from magnetic particles using RE1 solution. 8. Transfer samples away from magnetic beads to a new plate.

Flexible Purification
The Agencourt CosMCPrep kit can be used for purification of:
  • BACs
  • Cosmids
  • Fosmids
  • Low copy plasmid
  • High copy plasmid


Low Copy High Copy
Culture Volume (2xYT)   800µl 600µl
Yield   300ng-1.0µg 500ng-4.0µg
Phred20 Bases   600 650
Pass Rate*   88% 90%

*Passing Read: Average Phred value greater than 20 for bases between 100bp and 300bp.

Low and High Copy Plasmid Purification using a Single Protocol
One of the Agencourt CosMCPrep system's unique features is the ability to use a single process to purify both low and high copy plasmids in a fully automated format. Purified plasmids have exceptional sequencing performance with Phred 20 values routinely greater than 600 base pairs.

 



Figure 1: Nine pEPI-FOS5-based fosmids and nine pGEM-based plasmids were purified using identical Agencourt CosMCPrep protocols. Products were separated on a 1.2% agarose gel. A. Lane 1: MW ladder, Lane 2-10: P1-P9 plasmids. B. Lane 1: λ ladder, Lane 2-10: F1-F9 fosmids.
Sequencing Performance
Agencourt CosMCPrep-purified DNA, whether low or high copy in nature, produces high quality sequencing data. Agencourt has sequenced greater than 1,000,000 Agencourt CosMCPrep-purified, low copy number plasmids, including fosmids, BACS and low copy plasmids.

Figure 2 summarizes Phred20 read lengths from a Rickettsia genome sequencing project in which greater than 20,000 sequencing reads were performed on low copy plasmid templates. The average sequencing pass rate exceeded 90%, while Phred20 read lengths often exceeded 800 base pairs.

Agencourt CosMCPrep Automation
The Agencourt CosMCPrep protocol does not require the labor-intensive step of cellular debris removal, making it easier to automate the plasmid purification process. Automation of the Agencourt CosMCPrep protocol on medium or high-throughput robotic platforms has proven to quickly and efficiently purify high quality plasmids.

 


Figure 2: Low copy plasmids were sequenced using 1/16th BigDye1 v3.0. Unincorporated dye-terminators were removed using the Agencourt CleanSEQ kit and sequences read on an ABI 3700.

Available for use in your laboratory
To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.


Product Size Kit Components Product #
Agencourt CosMCPrep 4,000 rxn Kit 4,000 preps L2 Buffer
N3 Buffer
Pur4 Buffer
RE1 Buffer		 001046
Agencourt CosMCPrep 384 rxn Kit 384 preps L2 Buffer
N3 Buffer
Pur4 Buffer
RE1 Buffer		 A37064
spacer
Related Products Product #
Agencourt SPRIPlate® 96R - Ring Magnet Plate 000219
Please contact your sales representative or distributor with any questions. In the U.S. and Canada, call us at 800-361-7780. Outside the U.S. and Canada, call us at 978-867-2600. Email us at webinfo@agencourt.com.