The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood,serum and FTA cards. The kit is powered by cutting-edge Solid Phase Reversible Immobilization (SPRI®) paramagnetic bead-based technology to effectively produce a high recovery of DNA for downstream applications such as PCR¹ and genotyping. SPRI allows for fast separation,easy manipulation and simple automation compared to traditional centrifugation and vacuum filtration technologies. The method can be run manually in a 2 mL tube format or automated format on the Beckman Coulter Biomek® NXP or FXP workstations.

Application: Genomic DNA isolation from whole blood, plasma, serum, and FTA cards. Additional protocols are available for extracting genomic DNA from cultured eukaryotic cells, bacteria, and fresh or frozen tissue.*

Downstream Application Techniques: Genotyping applications (SNP, fragment analysis), clinical sequencing, resequencing, qPCR

Agencourt Genfind Features

  • Whole blood processing from 50 to 400 µL volumes
  • High gDNA recovery up to 6 µg from 200 µL of whole blood
  • No centrifugation or vacuum filtration required
  • Biomek Span-8 and 96-Multichannel methods are available for whole blood
  • Automated extraction of 96 samples in approximately 2.5 hours


1. Lyse whole blood or serum in Lysis Buffer and Proteinase K 2. Bind genomic DNA to paramagnetic beads 3. Separate beads from contaminants 4. Wash the magnetic beads with Wash Buffer 1 to remove contaminants 5. Wash the magnetic beads with Wash Buffer 2 to remove contaminants 6. Elute DNA from magnetic particles 7. Transfer to new plate

Consistent and High Recovery of gDNA
Figure 1 demonstrates that Agencourt Genfind v2 delivers high quality and consistent yields in comparison to competitor filtration and bead-based methods. The unique properties of the SPRI technology allows for the effective capture of gDNA in the presence of various anticoagulants.


Figure 1. Replicates of 8 200 µL samples of frozen human blood were manually extracted and eluted in 200 µL H2O (High Pure was eluted in 10 mM tris, pH 8.5); for all kits analyzed, aliquots were taken from the same patient sample for each anticoagulant used. DNA concentrations and yields were determined using the Beckman Coulter DTX plate reader to measure the A260 absorbance.

As seen in Table 1, Ct values obtained in qPCR reactions indicate that samples purified by the Agencourt Genfind v2 system produce amplifiable gDNA with a high sample-to- sample consistency.


Sample Mean Ct
Agencourt Genfind v2 10 ng 21.50 ± 0.37
MagneSil Blood Genomic Kit 10 ng 21.40 ± 0.15
QIAamp Blood Mini Kit 10 ng 20.18 ± 0.20
High Pure PCR Template Preparation Kit 10 ng 21.35 ± 1.03
Agencourt Genfind v2 1 ng 24.19 ± 0.17
MagneSil Blood Genomic Kit 1 ng 24.23 ± 0.48
QIAamp Blood Mini Kit 1 ng 22.96 ± 0.31
High Pure PCR Template Preparation Kit 1 ng 24.35 ± 0.77
Comparison of Ct values for nucleic acid purified using the Agencourt Genfind v2 system, MagneSil² Blood Genomic kit, QIAamp² Blood Mini kit, and High Pure² PCR Template Preparation kit. Indicated amounts of DNA manually extracted from frozen human blood prepared with EDTA were used in the qPCR analysis.


Figure 2. Gel electrophoresis of 1 µL genomic DNA through a 0.8% agarose gel. DNA was isolated from 200 µL blood with the Agencourt Genfind v2 purification method and eluted in 200 µL H2O. Key: M = 1 kb ladder, lanes 1 & 2 = Citrate, lanes 3 & 4 = EDTA, and lanes 5 & 6 = Heparin.

High Quality gDNA
The Agencourt Genfind v2 DNA isolation method isolates and purifies gDNA that is intact and suitable for downstream PCR and genotyping. Genomic DNA from human whole blood samples was isolated with Agencourt Genfind v2 and analyzed by gel electrophoresis (Figure 2).

Summary
The Agencourt Genfind v2 system is efficient and automation friendly. By automating the process using Agencourt software methods on either Biomek Span-8 or 96 Multichannel Biomek workstation, gDNA can be isolated and purified in approximately 2.5 hours. With the power of SPRI chemistry, the Agencourt Genfind v2 system produces highly pure DNA and consistent data thus allowing researchers to minimize retesting of precious samples.

Complete Automation
The use of the Agencourt SPRI paramagnetic bead-based technology allows Agencourt Genfind to be fully automated while maintaining a high sample quality. Genfind is currently optimized on Beckman Coulter's Biomek® FX, FXp, NX, and NXp Laboratory Automation Workstations using either a 96-well multichannel head or a Span-8 head.

Available for use in your laboratory
To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.


Product Size Kit Components Product #
Agencourt Genfind v2 DNA Isolation Tube Kit 50 preps Lysis Buffer
Wash I Buffer
Wash II Buffer
Binding Buffer
Proteinase K
Proteinase K Buffer
 A41499
Agencourt Genfind v2 DNA Isolation 96-well Plate Kit 384 preps
(4 x 96)		 Lysis Buffer
Wash I Buffer
Wash II Buffer
Binding Buffer
Proteinase K
Proteinase K Buffer
 A41497
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Related Products Product #
Software - Agencourt Genfind v2 96 MC - 3.x A42568
Software - Agencourt Genfind v2 96 - Biomek Span-8 - 3.x A42569
Software - Agencourt Genfind v2 FTA Cards 96 MC - v 3.x A46822
Agencourt AMPure® PCR Purification Kit (60 ml) A29152
Agencourt SPRIStand™ - Magnetic 6-tube Stand A29182
Agencourt SPRIPlate® Super Magnet Plate A32782
Please contact your sales representative or distributor with any questions. In the U.S. and Canada, call us at 800-361-7780. Outside the U.S. and Canada, call us at 978-867-2600. Email us at webinfo@agencourt.com.


1 The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.
2 All trademarks are property of their respective owners.
* Please inquire with your Agencourt sales representative for these additional protocols.