Agencourt offers high quality End Sequencing for BACs, cosmids, and fosmids with a throughput capacity of 25,000 end sequences per day. Our End Sequencing service provides:

• Rapid Turnaround
• Increased Linkage Rates
• Large Number of Phred20 Bases/Read
• High DNA Sequencing Pass Rates

 Learn more about our DNA Sequencing Pipeline

End Sequencing Includes: Low copy template purification with Agencourt CosMCPrep reagent Sequence reactions using BigDye Terminator v3.1 Dye terminator removal with Agencourt CleanSEQ kit Sequence delineation on an ABI PRISM 3730xl Base calling and data compilation Data delivery via secure FTP site Consistently High Pass Rates Agencourt's real time LIMS system allows continuous process control. Our attention to detail consistently produces high DNA sequencing pass rates for our customers. Figure 1 illustrates our average end sequencing pass rates, which are over 89%.		Figure 1: End sequencing pass rates for Agencourt's sequencing pipeline from 1/05 to 12/05 averaged above 89%.
High Linkage Rates Linkage of forward and reverse end sequences is dependent on both DNA sequencing pass rates and sample tracking (Figure 2). The combination of our high DNA sequencing pass rates and our exceptional sample tracking result in: Linkage rates over 95%, far exceeding those of other genomic service centers Fewer required reads per project Increased sample throughput Reduced project cost Proven Experience The Whitehead Institute for Biomedical Research acknowledged the throughput and quality of Agencourt's End Sequencing service by collaborating with us on projects to End Sequence 200,000 Ciona savignya and mouse fosmid clones. BAC Library Construction & Fingerprinting Agencourt provides researchers access to high quality BAC library construction services through Amplicon Express. Amplicon has built more than 500 BAC libraries of the highest quality with average insert sizes greater than 120 Kb. Libraries constructed at Amplicon Express are shipped directly to Agencourt for streamlined project completion. The combination of Amplicon Express' quality BAC libraries and Agencourt's high throughput DNA sequencing pipeline produce superior DNA sequencing results. Amplicon Express also offers clone arraying onto nylon filters, Pools & Superpools for PCR based screening, and BAC fingerprinting services. Find out more information about Amplicon Express BAC library Construction Services		Figure 2: Agencourt's linkage rates increase linearly with pass rates due to superior sample tracking practices. Figure 3: A BAC library was constructed at Amplicon Express from human tumor genomic DNA. Average insert size was calculated following enzyme digestion of 24 randomly chosen clones.