Agencourt offers a full length insert sequencing service for large insert vectors such as BACs, cosmids and fosmids. Agencourt has several features that allow us to provide high quality results with rapid turnaround:


 Learn more about our DNA Sequencing Pipeline

Full Length Insert Sequencing Includes:
  • Library construction
  • Template purification using Agencourt SPRI® reagents
  • DNA sequencing reactions using BigDye Terminator v3.1
  • Dye terminator removal with Agencourt CleanSEQ kit
  • Sequence delineation on an ABI PRISM 3730xl
  • Base calling and data compilation
  • Assembly with Phred, PHRAP and parcel genome assembler ARACHNE
  • Finishing, utilizing Consed with proprietary LIMS-integrated finishing software
  • Data delivery via secure FTP site

Library Construction
Agencourt uses unique library construction strategies to provide more random libraries with increased coverage. The approach to library construction is customized for the specific needs of each project and can include:
  • DNA shearing
  • Use of BstXI adapters
  • Creation of small and large insert sizes
  • Insert into high and/or low copy number vectors
  • Transcription free vectors

Finishing and Assembly
Agencourt uses both commercial and proprietary software for semi-automated finishing and assembly. Our finishing strategies can include:
  • Custom primer walking
  • Transposon insertion
  • Direct PCR sequencing


Assembly viewing and editing software. Inset: Aligned sequences of individual project clones represented as horizontal bars. Background: Individual DNA sequencing electropherograms that are easily viewed for quality assessment.

Results
Results are delivered via a secure FTP site. Data delivery is personally overseen by a member of the Agencourt staff to ensure simple and timely results transfer. Compiled data is available in multiple file formats including:
  • FASTA
  • .AB1
  • .SCF
  • .QUAL