Agencourt Bioscience, the leading commercial DNA Sequencing services provider, is offering a new portfolio of genomic services to include massively parallel sequencing technologies. Utilizing a cross platform strategy to merge the strengths of various platforms, we are now offering services on the Roche 454 GS-FLX¹ and Applied Biosystems SOLiD¹ platforms. By offering these new technologies, we can utilize new applications and hence expand the tools available to our customers. We have the ability to harness the power of the technology and increase cost savings by using next generation sequencing. Agencourt's value-added services and packaged solutions provide a strong complement to these new innovations.


Our complementary services can provide:


  • Upstream fragment library construction for various insert sizes and applications
  • Custom and novel cDNA library construction and pooling techniques
  • Integration of our patented SPRI® magnetic bead sample prep methodology
  • Streamlined and standardized sample prep on our Biomek® FX and automation solutions
  • Integration and secured management of customer data using our custom Oracle-based Laboratory Information Management Systems (LIMS)
  • Complementary bioinformatics services for assembly, data packaging and analysis
  • Excess Sanger sequencing capacity for Genome finishing and hybrid strategies
  • Genome Annotation and Pathway reconstruction using ERGO¹

Key technology features:


  • Obtain up to 100Mb or 3Gb of easy-to-map data per run
  • Single molecule or clonal amplification of templates to avoid cloning bias
  • Ability to run up to 16 samples on separate channels
  • Mated paired end libraries
  • 35 to 250 bp of high quality read lengths depending on the platform and application
  • Robust chemistry for accurate base-calling

Next Generation Sequencing Applications


Whole Genome Analysis Whole Genome Analysis
  1. High quality finished genome via Sanger sequencing
  2. Hybrid sequencing strategies
  3. Massively parallel sequencing
  4. ERGO gene annotation and metabolic pathway analysis

Transcriptome Analysis Transcriptome Analysis
  1. Custom or normalized cDNA library construction
  2. EST or SAGE Sequencing
  3. Massively parallel sequencing of pooled cDNA libraries or genomic tags

RNA Analysis RNA Analysis
  1. 16s RNA sequencing
  2. Micro RNA sequencing
  3. Massively parallel sequencing of tags

Structural variation analysis Structural Variation Analysis
  1. Fosmid or BAC library construction
  2. Mapping INDELS and re-arrangements
  3. Massively parallel sequencing of tags

¹ Trademarks are property of their respective owners.