Agencourt offers a Primer Walking sequencing service for PCR1 amplicons, plasmid inserts, and entire plasmids to produce single strand or publication quality coverage. Our Primer Walking service is powered by Agencourt's GLP-compliant, world class sequencing facility, with the capacity to process over 45 million Phred20 bases per day. The key features of our Primer Walking sequencing service are:

  • Fully automated, GLP-compliant facility
  • Galaxy LIMS
  • Sophisticated bioinformatics
  • Proprietary primer design software
  • Highly experienced scientists


Figure 1: Agencourt follows the Bermuda standard for quality assessment for publication quality coverage.

Primer Walking Service Includes:

  • Sequence reactions using BigDye Terminator v3.1
  • Sequence detection on ABI 3730xl DNA sequencers
  • Dye terminator removal using Agencourt CleanSEQ reagent
  • Base calling and quality scoring
  • DNA sequence finishing and assembly
  • Personal service from our dedicated project managers
  • Data delivery via secure FTP site

Flexibility and Convenience
Agencourt serves as an extension of your laboratory. We are able to tailor our service offerings to meet your specific needs.

Quality
The combination of the Agencourt SPRI ® chemistry and our world class pipeline produce long Phred20 read lengths and high DNA sequencing pass rates. Agencourt uses the industry gold standard for quality assessment, the Bermuda standard (Phrap 40), for publication quality coverage.

Data Integrity
Our facility was developed to maximize data integrity and security. Security features include:

  • Secure ftp site
  • Complex password system
  • Thorough disaster recovery measures
  • Full system redundancy
  • Complete bar coded pipeline
  • Proprietary Galaxy LIMS


Figure 2: All samples are bar coded and tracked throughout our pipeline to ensure complete data security.

Results
Results are delivered via a secure FTP site. Data delivery is personally overseen by a member of the Agencourt staff to ensure simple and timely results transfer. Compiled data are available in multiple file formats including:

  • FASTA
  • .AB1
  • .SCF
  • .QUAL

2D Bar Coded Sample Tubes
Polypropylene tubes with integrated 2D bar code designed for research sequencing sample submission. They are available in packages of 96 tubes.


Product Product #
2D Bar Coded Sample Tubes 001108
For all other inquiries contact your sales representative or distributor. In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.

To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2603. Email us at webinfo@agencourt.com.

1 The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.