Agencourt offers quick turnaround DNA sequencing of individual research samples, including PCR1 amplicons, low copy plasmids, and high copy plasmids. The QuickLane DNA sequencing service is powered by Agencourt's GLP-compliant, high performance DNA sequencing pipeline, which processes over 20 million Phred20 bases per day. The power of this pipeline is now available to researchers with single samples. The key benefits of Agencourt's QuickLane DNA sequencing service are:

  • 24-hour sample turnaround
  • Long reads on our ABI 3730xl DNA sequencers
  • Free universal primers
  • Sample preparation (optional)
  • Industry leading sequence quality
  • High-level sample tracking
  • Personal service from our dedicated project managers

Find out more information about what makes Agencourt's DNA sequencing pipeline unique.

Fast, Reliable Sequencing
We understand that every sample you submit is precious. We take extreme care to ensure the highest level of sample tracking and data quality for every sample.

Sample Tracking & Quality Assessment
Agencourt has the most sophisticated LIMS system in the industry. Quality control is monitored and managed at all levels of our genomic pipeline. Our state-of-the-art Oracle-based LIMS tracks sample performance, well-to-well genealogy, instrument calibration, instrument performance, reagent quality, and assay data from the moment your samples enter Agencourt to the time we ship your data.

2D Bar Coded Tubes
Figure 1: 2D bar coded tubes enable us to track your samples as they move through the DNA sequencing pipeline.
Real Time Sample Trackings
Figure 2: Sample tracking is fully automated using our Galaxy LIMS. Well location, quality performance parameters and sample data are gathered at every stage in our pipeline in real time using bar coded plates and tubes and scanners to ensure data accuracy.

High Quality Sequence Data
Whether you are sequencing a single plate or a single sample, the power of Agencourt's proven genomics pipeline, SPRI® technology technology and Galaxy LIMS produce the highest quality sequence data available. Our DNA sequencing pipeline pass rates routinely exceed 85%. Long read lengths are generated using our ABI 3730xl sequencers. The NHGRI recently acknowledged Agencourt's dedication to quality and security by appointing us as the only commercial Large-Scale Sequencing Center responsible for sequencing genomes of high biomedical importance.

Samples
Samples for quick turnaround sequencing must be submitted in 2D Bar Coded Tubes or 96-well microtiter plates. DNA and primers can be submitted pre-mixed or independently.

Agencourt provides the following universal primers free:

  • Universal T7
  • M13-FWD
  • M13-REV
  • SP6
  • Universal T3
  • KBR/TJ
  • 2BP Clamp
  • SeqL-A
  • SeqL-B
  • T7 Terminator
  • CMV Forward
  • BGH Reverse

Please see our Sample Submission Guidelines for more information.

  


Figure 3: An electropherogram of a BAC purified using CosMCPrep® and sequenced using BigDye® Terminator v3.1. The sequencing reaction was purified using CleanSEQ® and run on an ABI 3730.

Results
Results are delivered via a secure FTP site. Data delivery is personally overseen by a member of the Agencourt team to ensure simple and timely results transfer. Compiled data are available in multiple file formats including:
  • FASTA
  • .AB1
  • .SCF
  • .QUAL

2D Bar Coded Sample Tubes
Polypropylene tubes with integrated 2D bar code designed for research sequencing sample submission. They are available in packages of 96 tubes.


Product Product #
2D Bar Coded Sample Tubes 001108
For all other inquiries contact your sales representative or distributor. In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2600. Email us at webinfo@agencourt.com.

To Place an Order or Receive Technical Assistance: In the U.S. and Canada call us at 800-361-7780. Outside the U.S. and Canada call us at 978-867-2603. Email us at webinfo@agencourt.com.

1 The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.