Agencourt's SNP Discovery and Resequencing service packages produce quality identification of SNPs, insertions and deletions. These services use a targeted resequencing approach for accurate polymorphism identification. Agencourt's facility, high throughput pipeline, and our DNA sequencing expertise set us apart as a premier SNP service provider able to handle even the largest of projects.


Quality Polymorphism Identifications

Unmatched Assay Design & Validation
Agencourt uses fully-automated and optimized assay design software for amplicon modeling. Our unique assay validation process produces amplicon success rates that average over 90%. All amplicons are PCR and sequence-validated prior to full project screening. Reactions that do not pass undergo further rounds of assay development using techniques such as alternative protocols, additives, nested reactions, and redesigned primer sets. Our assay development experience allows us to design assays spanning genomic regions of varying degrees of sequence difficulty.

Quality Amplification & Sequencing
PCR amplification reactions are set up in a dedicated clean room using fully-automated robotic platforms. All samples undergo bi-directional DNA sequencing to increase quality and resolve homopolymers, insertions, deletions and microsatellites.

Flexible Analysis Packages
Agencourt has optimized the latest version of PolyPhred to produce highly accurate SNP identification with low false positive rates and low false negative rates. We have developed a proprietary algorithm to deploy different PolyPhred stringencies for specific sequence contexts in any given amplicon. This dynamic PolyPhred deployment has increased the PolyPhred accuracy to above 90%. PolyPhred analysis, even with optimization, has limitations identifying insertions and deletions. Agencourt offers manual analysis of these automated reports to eliminate any remaining false positives and to identify insertions and deletions. Basic manual analysis includes identification of insertion and deletion start sites, while advanced manual analysis also includes annotation of all inserted and deleted sequences within each sample.

Agencourt's proprietary SNP Scoring Software allows rapid identification of:

  • Intronic and exonic SNPs
  • Amino acid changing SNPs
  • dbSNP novelty of SNPs
  • Patient genotypes

The Agencourt Edge:
  • Assay design with cutting edge proprietary software
  • Validation & optimization for higher amplicon success rates
  • High throughput DNA sequencing capacity for large-scale SNP identification
  • Automated & manual analysis packages for accurate and cost effective identification of SNPs, insertions, and deletions
  • Pre-validated kinase & oncogene assays for rapid project turnaround




Full Validation For Increased Assay Success



Proprietary SNP Scoring Screenshot

Automated software annotates both previously known (dbSNP) and newly discovered SNPs. Intron and exon boundries are annotated in addition to any amino acid changing SNPs and the respective amino acid change.