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AMPure Publications
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A Versatile Ligation-Independent Cloning Method Suitable For High-Throughput Expression Screening Applications
NS Berrow, D Alderton, S Sainsbury, J Nettleship - Nucleic Acids Research, 2007 - Oxford Univ Press
... All other PCR products were purified using AMPure magnetic beads (Beckman-Coulter, High Wycombe, Buckinghamshire, UK) according to the manufacturer's ...
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High-Throughput Species Identification: From DNA Isolation To Bioinformatics
DE RICHARDSON, JD VANWYE, AMYM EXUM, RK COWEN, DL - Molecular Ecology Notes, 2007 - ingentaconnect.com
... PCR products were then purified using the standard AmPure kit (Agencourt) protocol on the liquid- handling robot, but with a 1:1 ratio of magnetic beads to PCR ...
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The Genetic Spectrum Of A Population-Based Sample Of Familial Hemiplegic Migraine
LL Thomsen, M Kirchmann, A Bjornsson, H Stefansson - Brain, 2007 - Oxford Univ Press
... TM . PCR products were verified for correct length on an agarose gel before being purified using AMPure TM from Agencourt. Cycle ...
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DNA Barcoding Using Chitons (Genus Mopalia)
RP KELLY, IN SARKAR, DJ EERNISSE, ROB DESALLE - Molecular Ecology Notes, 2007 - ingentaconnect.com
... PCR products were cleaned using either 96-well filter plates (Millipore Corp.) or AMPure beads (Agencourt Corp., protocols available from manufacturer). ...
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Casting the Percomorph Net Widely: The Importance of Broad Taxonomic Sampling in the Search for the Placement of Serranid and Percid Fishes
WL Smith, MT Craig, JM Quattro - Copeia - bioone.org
... were desalted and concentrated using an ArrayIt PCR Product Purification ... modifications to the manufacturer's protocol or using AMPure (Agencourt Biosciences)...
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Epidermal Growth Factor Receptor Mutation Testing in the Care of Lung Cancer Patients
LV Sequist, VA Joshi, PA Janne, DW Bell, P Fidias - Clinical Cancer Research, 2006 - AACR
... 1 minute for 30 cycles; and 72°C for 10 minutes. PCR products are purified with AMPure magnetic beads (Agencourt, Beverly, MA). ...
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Funnybase: A Systems Level Functional Annotation Of Fundulus Ests For The Analysis Of Gene Expression
JE Paschall, MF Oleksiak, JD VanWye, JL Roach, JA - feedback, 2006 - biomedcentral.com
... PCR products were purified manually in 96 well format using Sephadex ... 0.2 microfilter (Millipore, Billerica, USA) or robotically using AmPure (Agencourt, Beverly ...
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Bacteria In Oral Secretions Of An Endophytic Insect Inhibit Antagonistic Fungi
F Password, E Entomology - Ecological Entomology, 2006 - Blackwell Synergy
... 2004. PCR products were purified using AMPure magnetic beads (Agencourt Bioscience Corp., Beverly, Massachusetts). Bacterial isolates ...
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Phylogenetics of the florally diverse Andean clade Iochrominae (Solanaceae)
SDW Smith, DA Baum - American Journal of Botany, 2006 - Botanical Soc America
... min. All PCR products were purified with AMPure using manufacturer's protocols (Agencourt Bioscience, Beverly, Massachusetts, USA). ...
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Phylogenetic Relationships Of South American Lizards Of The Genus Stenocercus (Squamata: Iguania): A New Approach Using A General Mixture Model For Gene Sequence Data
O Torres-Carvajal, JA Schulte, JE Cadle - Molecular Phylogenetics and Evolution, 2006 - puce.edu.ec
... were puriWed with either spin puriWca- tion columns (Qiagen, Inc.) or AMPure magnetic beads ... PCR and sequencing primers used in this study are listed in Table 2 ...
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Phylogeny And Biogeography Of Croton Alabamensis (Euphorbiaceae), A Rare Shrub From Texas And Alabama, Using DNA Sequence And AFLP Data
BW VAN EE, N JELINSKI, PE BERRY, AL HIPP - Molecular Ecology, 2006 - Blackwell Synergy
... Polymerase chain reaction (PCR) products were cleaned using the AMPure magnetic bead method and sequenced at the University of Wisconsin Biotechnology Center ...
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RFLP Analysis Of The Mtdna Control Region In White Shrimp (Litopenaeus vannamei) populations from the eastern Pacific
R Valles-Jimenez, PM Gaffney, R Perez-Enriquez - Marine Biology, 2006 - Springer
... The PCR prod- ucts were cleaned with a magnetic purification kit (AMPure, Agencourt Bioscience Corporation) following the manufacturer's protocols. ...
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Germline Gain-Of-Function Mutations In SOS1 Cause Noonan Syndrome
AE Roberts, T Araki, KD Swanson, KT Montgomery, TA - Nature Genetics, 2006 - nature.com
... PCR products were purified using AMPure beads (Agencourt) and then sequenced using ABI BigDye 3.1 dye terminator chemistry and a 3730XL DNA analyzer.
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Basilar-Type Migraine Clinical, Epidemiologic, And Genetic Features
M Kirchmann, LL Thomsen, J Olesen - Neurology, 2006 - AAN Enterprises
... Tetrad. PCR products were verified for correct length on agarose gel before being purified using AMPure from Agencourt...
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Atypical Haemolytic Uraemic Syndrome Associated with a Hybrid Complement Gene
JP Venables, L Strain, D Routledge, D Bourn, HM ... - PLoS Med, 2006 - medicine.plosjournals.org
... PCR products were purified using magnetic microparticles (AMPure, Beckman) to remove unincorporated dNTPs, primers, and salts. Sequencing ...
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On The Wegener Granulomatosis Associated Region On Chromosome 6 P 21. 3
P Szyld, P Jagiello, E Csernok, WL Gross, JT ... - BMC Medical Genetics, 2006 - biomedcentral.com
... PCR conditions were: 1 x PCR buffer (Qiagen), 0.2 mM each dNTP, 0.2 pmol ... for 10 min. The product was purified with magnetic beads system (AMPure...
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Characterization of Gut-Associated Bacteria in Larvae and Adults of the Southern Pine Beetle, Dendroctonus frontalis Zimmermann
A Vasanthakumar, I Delalibera, J Handelsman, KD ... - Environmental Entomology - bioone.org
... final extension at 72°C for 5 min. PCR products were purified using AMPure magnetic beads (Agencourt Bioscience, Beverly, MA). ...
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Molecular Phylogeography Of The Fruit Bat Genus Melonycteris In Northern Melanesia
JN Pulvers, DJ Colgan - Blackwell Synergy
... COI PCR products were purified manually using AMPURE magnetic beads (Agencourt, Beverly, MA, USA). Generally, products were sequenced in both directions. ...
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Protist Genetic Diversity in the Acidic Hydrothermal Environments of Lassen Volcanic National Park, USA
PB BROWN, GV WOLFE - Journal of Eukaryotic Microbiology, 2006 - Blackwell Synergy
... PCR primers were removed from PCR-amplified SSU rRNA genes with AMPure magnetic beads (Agencourt Biosciences, Beverly, MA) following the manufacturer's ...
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Early Detection And Progression Of Decay In L-Joints And Lap-Joints In A Moderate Decay Hazard Zone
CA Clausen, TL Highley, DL Lindner - FOREST PRODUCTS JOURNAL - fpl.fs.fed.us
... minutes. The PCR products were cleaned using AmPure magnetic beads (Agencourt) following manufacturer's direc tions. Sequencing ...
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Evolutionary History Of Wolbachia Infections In The Fire Ant Solenopsis Invicta
ME Ahrens, D Shoemaker - feedback, 2006 - biomedcentral.com
... Wsp PCR amplicons were purified for sequencing using AMPure magnetic beads (Agencourt Bioscience Corp.) and subsequently used directly in standard fluorescent ...
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High-Throughput Species Identification: From DNA Isolation To Bioinformatics
DE RICHARDSON, JD VANWYE, A EXUM, RK COWEN, DL ... - Blackwell Synergy
... PCR products were then purified using the standard AmPure kit (Agencourt) protocol on the liquid-handling robot, but with a 1:1 ratio of magnetic beads to PCR ...
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Aberrant Promoter Methylation And Silencing Of The POU2F3 Gene In Cervical Cancer
Z Zhang, PC Huettner, L Nguyen, M Bidder, MC Funk, ... - Oncogene, 2006 - nature.com
... The PCR conditions were: 95°C for 3 min, 94°C for 30 s ... Amplified products were purified using AMPure (Agencourt Bioscience Corp., Beverly, MA, USA), and were ...
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Preliminary Assessment Of Population Structure In The Mackerel Icefish (Champsocephalus gunnari)
KL Kuhn, PM Gaffney - Polar Biology, 2006 - Springer
... PCR products were then 928 Page 3. purified for sequencing using AMPure beads according to manufacturer's protocol (Agencourt Bioscience, Beverly, MA). ...
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De Novo Gene Conversion In The RCA Gene Cluster (1q32) Causes Mutations In Complement Factor H Associated With Atypical Hemolytic Uremic Syndrome
S Heinen, P Sanchez-Corral, MS Jackson, L Strain, ... - Hum Mutat, 2006 - doi.wiley.com
... PCR products were purified using magnetic microparticles (AMPure, Agencourt Biosciences) to remove unicorporated dNTPs, primers and salts. ...
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Strategies For Evaluating B*18 Allelic Diversity By Sequence-Based Typing Applied To Studies Of A Population From Singapore And African-Americans
L Hou, B Tu, G Ling, T Tang, K Cao, NK Steiner, A ... - Tissue Antigens, 2006 - ingentaconnect.com
... approximately 1100 bp amplicon using AMPure according to the manufac- turer's instructions (Agencourt, Beverly, MA), direct sequencing of the PCR product was ...
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Genetic Testing In Atypical HUS And The Role Of Membrane Cofactor Protein (MCP; CD46) And Factor I
THJ Goodship, V Fremeaux-Bacchi, JP Atkinson - 2005 - Springer
... 1-3]. PCR products are purified using magnetic microparticles (AMPure, Agencourt Biosciences) to remove unincorporated dNTPs, primers and salts. ...
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Fine Mapping And Evaluation Of Candidate Genes For Cervical Cancer On 11 Q 23
Z Zhang, DS Gerhard, L Nguyen, J Li, A Traugott, - Genes Chromosomes and Cancer, 2005 - doi.wiley.com
... For direct sequencing (POU2F3 and TRIM29), we separated PCR products by agarose gel electro- phoresis and purified them with AMPure (Agencourt, Beverly, MA). ...
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Conversion Analysis for Mutation Detection in MLH1 and MSH2 in Patients With Colorectal Cancer
G Casey, NM Lindor, N Papadopoulos, SN Thibodeau, - 2005 - Am Med Assoc
... Sequencing of cDNA Products. The PCR fragments were purified using the AMPure purification system (Agencourt, Beverly, Mass). Sequencing ...
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Evolution And Diversification Of A Sexually Dimorphic Luminescent System In Ponyfishes (Teleostei: Leiognathidae), Including Diagnoses For Two New Genera
JS Sparks, PV Dunlap, WL Smith - Cladistics, 2005 - Blackwell Synergy
... stranded amplification products were either desalted and concentrated using Qiagen Quick-Spin PCR Purification Columns, using AMPure (Agencourt Biosciences ...
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b-Catenin (CTNNB1) Gene Amplification: A New Mechanism of Protein Overexpression in Cancer
G Suriano, N Vrcelj, J Senz, P Ferreira, H Masoudi - GENES, CHROMOSOMES & CANCER, 2005 - doi.wiley.com
... PCR products were purified with AmPure mag- netic beads (AgencourtBioscience, Beverly, MA), and a 2-ml aliquot of purified PCR product was then cycle-sequenced ...
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Association of NEDD4L Ubiquitin Ligase With Essential Hypertension
CJ Russo, E Melista, J Cui, AL DeStefano, GL ... - 2005 - Am Heart Assoc
... DNA sequencing was performed by polymerase chain reaction (PCR) amplification of the target exon, purification of the product (AMPure solid-phase reversible ...
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Molecular Phylogenetics And Patterns Of Floral Evolution In The Ericales
J Schoenenberger, AA Anderberg, KJ Sytsma - International Journal of Plant Sciences, 2005 - journals.uchicago.edu
... (1998). We cleaned PCR products using AMPure magnetic particles (Agencourt Bioscience, Beverly, MA)...
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Analyzing Ligation Mixtures Using A PCR Based Method
PK Chandra, SK Wikel - Biol. Proced. Online, 2005 - biologicalprocedures.com
... 1). The PCR products were purified using the AMPure® solid-phase paramagnetic beads (Agencourt Biosciences, Beverly, MA, USA) and used for TA cloning into pCR ...
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A Deletion Causing Spontaneous Fracture Identified From A Candidate Region Of Mouse Chromosome 14
Y Jiao, X Li, WG Beamer, J Yan, Y Tong, D ... - Mammalian Genome, 2005 - Springer
... To verify the mutated gene (LGO) and the dele- tion, PCR products from both genomic and cDNA were purified using an AMPure PCR purification kit (Agencourt ...
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P53-Independent NOXA Induction Overcomes Apoptotic Resistance Of Malignant Melanomas
JZ Qin, L Stennett, P Bacon, B Bodner, MJC Hendrix - Molecular Cancer Therapeutics, 2004 - AACR
... was amplified and PCR reactions were purified after thermocycling using solid-phase reversible immobilization-based technology (AMPure, Agencourt Biosciences ...
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Abnormal Cerebellar Development And Axonal Decussation Due To Mutations In AHI1 In Joubert Syndrome
RJ Ferland, W Eyaid, RV Collura, LD Tully, RS Hill - Nature Genetics, 2004 - nature.com
... and purified them using either the PSI Clone PCR 96 kit (Princeton Separations) or the AMPure kit (Agencourt) in accordance with the manufacturer's protocols...
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Novel Mutations, No Detectable Mrna And Familial Genetic Analysis Of The Wiskott-Aldrich Syndrome Protein Gene In Six Japanese Patients With Wiskott-Aldrich Syndrome
Y Sasahara, S Kawai, S Kumaki, Y Ohashi, M - European Journal of Pediatrics, 2000 - Springer
... to direct sequencing analysis with the same primers used for the PCR amplifcation ...The IgG fraction was obtained with Protein A column AMPure PA kit...
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Generation of Polymeric Immunoglobulin Receptor-Deficient Mouse with Marked Reduction of Secretory IgA
S Shimada, M Kawaguchi-Miyashita, A Kushiro, T - The Journal of Immunology, 1999 - Am Assoc Immnol
... Screening was conducted by PCR using the following primers: sense, 5'-ATCTCGTCGTGACCCATGGCGATGC ... serum was recovered and IgG fraction purified with AMPure ...
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Reproducibility of Gene Expression Signature-Based Predictions in Replicate Experiments
K Anderson, KR Hess, M Kapoor, S Tirrell, J - 1539 - AACR
... fragmentation buffer. The robotic cDNA and cRNA purification used AMPure PCR purification System (Agencourt, Beverly, MA). Fragmented cRNA ...
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Germline E-Cadherin Mutations In Hereditary Diffuse Gastric Cancer: Assessment Of 42 New Families And Review Of Genetic Screening Criteria
SG Seruca, C Caldas, D Huntsman, SN Gilpin, D - British Medical Journal - jmg.bmj.com
... The remaining 17 ml of PCR product was purified using AmPure magnetic beads (Agencourt Bioscience, Beverly, MA)...
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Southern Hospitality: A Latitudinal Gradient In Gene Flow In The Marine Environment
F Password - Blackwell Synergy
... PCR products were cleaned using either 96-well filter plates (Millipore Corp., Billerica, MA) or AmPure beads (Agencourt Corp. Beverly, MA)...
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Phylogeographic Structure Of The Fire Ant Solenopsis Invicta In Its Native South American Range: Roles Of Natural Barriers And Habitat Connectivity
ME Ahrens, KG Ross, DDW Shoemaker - Evolution - bioone.org
... DNA amplicons were purified using Agencourt (Beverly, MA) AMPure magnetic beads and used directly in standard fluorescent cycle-sequencing PCR reactions
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DNA barcoding using chitons (genus Mopalia)
ME Notes - Blackwell Synergy
... PCR products were cleaned using either 96-well filter plates (Millipore Corp.) or AMPure beads (Agencourt Corp., protocols available from manufacturer). ...
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The BARD1 Cys557Ser Variant and Breast Cancer Risk in Iceland
SN Stacey, P Sulem, OT Johannsson, A Helgason, J - medicine.plosjournals.org
... PCR products were verified for correct length by agarose gel electrophoresis and purified using AMPure (Agencourt Bioscience; http:/ / www.agencourt.com). ...
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