Agencourt Bioscience | CleanSEQ Publications

CleanSEQ Publications

Methods Of Producing And Sequencing Modified Polynucleotides DR Smith, KJ McKernan - 2007 - freepatentsonline.com ... a cleavage buffer, pyrophosphate, one or more supports for isolating extension products, one or more reagents for sample clean-up (eg, CleanSEQ, AmPure) and ...
5-Aza-2'-deoxycytidine-Mediated Reductions in G9A Histone Methyltransferase And Histone H3 K9 Di-Methylation Levels Are Linked To Tumor Suppressor Gene Reactivation RJ Wozniak, WT Klimecki, SS Lau, Y Feinstein, BW ... - Oncogene, 2007 - nature.com ... These reactions were further processed by running a clean-up procedure with CleanSEQ beads (Agencourt Bioscience Corporation, Beverly, MA, USA) on a Biomek FX ...
The Genetic Spectrum Of A Population-Based Sample Of Familial Hemiplegic Migraine LL Thomsen, M Kirchmann, A Bjornsson, H Stefansson ... - Brain, 2007 - Oxford Univ Press ... Cycle sequencing reactions were set up on Sciclone ALH 300, run on MJR Tetrad TM and excess dye terminators were removed using CleanSEQ TM from Agencourt. ...
High-throughput species identification: from DNA isolation to bioinformatics DE RICHARDSON, JD VANWYE, AMYM EXUM, RK COWEN, DL ... - Molecular Ecology Notes, 2007 - ingentaconnect.com ... Sequences were purified on the liquid-handling robot using the standard CleanSEQ kit protocol (Agencourt) and then run on a 3730 xl DNA Analyser (Applied ...
Sensitivity analysis, molecular systematics and natural history evolution of Scathophagidae (Diptera: Cyclorrhapha: Calyptratae) F Password - Cladistics, 2007 - Blackwell Synergy ... using BigDye Terminator v3.1. The products were then prepared for direct sequencing by removing the dye-terminator using 5 µL of Agencourt CleanSEQ solution. ...
The Wiscdslox T-DNA Collection: An Arabidopsis Community Resource Generated By Using An Improved High-Throughput T-DNA Sequencing Pipeline ST Woody, S Austin-Phillips, RM Amasino, PJ Krysan - Journal of Plant Research, 2007 - Springer ... Unincorporated dyes were removed from cycle- sequencing reactions by using paramagnetic beads (CleanSEQ; Agencourt, Beverly, MA, USA) to sequester extension ...
Augmentation of NZB Autoimmune Phenotypes by the Sle1c Murine Lupus Susceptibility Interval BM Giles, SN Tchepeleva, JJ Kachinski, K Ruff, BP ... - The Journal of Immunology, 2007 - Am Assoc Immnol ... dideoxynucleotides (dye terminators) were removed from the cycle-sequencing reaction products using the paramagnetic bead technology (CleanSEQ, part number ...
Novel Somatic and Germline Mutations in Cancer Candidate Genes in Glioblastoma, Melanoma, and Pancreatic Carcinoma A Balakrishnan, FE Bleeker, S Lamba, M Rodolfo, M ... - Cancer Research, 2007 - AACR ... Sequencing products were purified using CleanSEQ (Agencourt Bioscience, Beckman Coulter) and analyzed on a 3730 DNA Analyzer, ABI capillary electrophoresis ...
Autosomal Recessive Corneal Endothelial Dystrophy (CHED2) Is Associated With Mutations In SLC4A11 X Jiao, A Sultana, P Garg, B Ramamurthy, GK ... - British Medical Journal, 2007 - jmg.bmj.com ... reactions were purified using the Edge Biosystem Performa TM DTR Gel Filtration System (Gaithersburg, Maryland, USA) or AmPure and CleanSEQ reagents (Agencourt ...
Evolutionary History Of Wolbachia Infections In The Fire Ant Solenopsis Invicta ME Ahrens, D Shoemaker - feedback, 2006 - biomedcentral.com ... Sequencing reactions were purified using CleanSEQ magnetic beads (Agencourt Bioscience Corp.) and run on an ABI 3700 sequencer at the UW Biotechnology Center ...
Construction Of A Nurse Shark (Ginglymostoma Cirratum) Bacterial Artificial Chromosome (BAC) Library And A Preliminary Genome Survey M Luo, HR Kim, D Kudrna, NB Sisneros, SJ Lee, C ... - BMC Genomics, 2006 - pubmedcentral.nih.gov ... of 10 sec at 95°C, 5 sec at 55°C, and 2.5 min at 60°C. After the cycle-sequencing step, the DNA was purified by magnetic beads, CleanSEQ (Agencourt, Beverly ...
The Oryza Bacterial Artificial Chromosome Library Resource: Construction And Analysis Of 12 Deep-Coverage Large-Insert BAC Libraries That Represent The 10 Genome Types Of The Genus Oryza JSS Ammiraju, M Luo, JL Goicoechea, W Wang, D ... - Genome Research, 2006 - Cold Spring Harbor Lab ... using the following conditions: 150 cycles of 10 sec at 95°C, 5 sec at 55°C, and 2.5 min at 60°C, followed by DNA purification using CleanSEQ (Agencourt). ...
Funnybase: A Systems Level Functional Annotation Of Fundulus ESTs For The Analysis Of Gene Expression JE Paschall, MF Oleksiak, JD VanWye, JL Roach, JA ... - feedback, 2006 - biomedcentral.com ... Sequences were purified using biotin primers and streptavidin coated magnetic beads (for the ABI 373) or Agencort CleanSEQ (for the ABI 3730). Validation. ...
A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture MS Pischke, EL Huttlin, AD Hegeman, MR Sussman - PLANT PHYSIOLOGY, 2006 - 171.66.122.39 ... For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli...
Method To Determine Responsiveness Of Cancer To Epidermal Growth Factor Receptor Targeting Treatments DW Bell, DA Haber, PA Janne, BE Johnson, TJ Lynch, ... - 2006 - freepatentsonline.com ... The present invention is directed to a method for determining the responsiveness of cancer to an epidermal growth factor receptor (EGFR) treatment...
Phylogeny Of South American Bufo (Anura: Bufonidae) Inferred From Combined Evidence OFSAB PHYLOGENY, B PRAMUK - ingentaconnect.com ... Despite the considerable research that has focused on the evolutionary relationships and biogeography of the genus Bufo, an evolutionary synthesis of the entire group...
Atypical Haemolytic Uraemic Syndrome Associated With A Hybrid Complement Gene JP Venables, L Strain, D Routledge, D Bourn, HM ... - PLoS Med, 2006 - medicine.plosjournals.org ... were carried out by dye terminator cycle sequencing (DTCS kit, Beckman) using UNISEQ primers, purified using magnetic microparticles (CleanSEQ, Beckman), and ...
Genetic Signature Of Recent Glaciation On Populations Of A Near-Shore Marine Fish Species (Syngnathus leptorhynchus) AB WILSON - Molecular Ecology, 2006 - ingentaconnect.com ... min). Sequencing reactions were purified with CleanSEQ magnetic beads (Agencourt) following the manufacturer's recommendations. ...
A Bacterial Artificial Chromosome Library For Biomphalaria Glabrata, Intermediate Snail Host Of Schistosoma mansoni CM Adema, MZ Luo, B Hanelt, LA Hertel, JJ Marshall ... - Memrias do Instituto Oswaldo Cruz, 2006 - SciELO Brasil ... in 384-well format applying 150 cycles of 10 s at 95°C, 5 s at 55°C, and 2.5 min at 60°C. Extension products were purified by CleanSEQ magnetic beads ...
Early detection and progression of decay in L-joints and lap-joints in a moderate decay hazard zone CA Clausen, TL Highley, DL Lindner - FOREST PRODUCTS JOURNAL - fpl.fs.fed.us ... ITS4 primer. Sequencing reactions were cleaned using CleanSEQ (Agen court) according to manufacturer's directions. Sequencing ...
Molecular phylogeny of Cucumis species as revealed by consensus chloroplast SSR marker length and sequence variation SM Chung, JE Staub, JF Chen - Genome, 2006 - ingentaconnect.com ... The dye terminators were then removed using the CleanSEQ R (Agencourt, Beverly, Mass) system, followed by sequencing of reactions. ...
A High-Throughput Gene Knockout Procedure For Neurospora Reveals Functions For Multiple Transcription Factors HV Colot, G Park, GE Turner, C Ringelberg, CM Crew ... - Proceedings of the National Academy of Sciences, 2006 - National Acad Sciences ... with slight modifications: Zymolyase T-100 (Seikagaku America, Falmouth, MA) was used and, in place of the final precipitation step, CleanSEQ magnetic beads ...
Genetic Variation and Selection Response in Model Breeding Populations of Brassica rapa Following a Diversity Bottleneck WH Briggs, IL Goldman - Genetics, 2006 - Genetics Soc America ... Final PCR fragments were purified with CleanSEQ magnetic beads (Agencourt, Beverly, MA) and mixed with GeneFlo 625 Rox-labeled internal size standards...
Protist Genetic Diversity in the Acidic Hydrothermal Environments of Lassen Volcanic National Park, USA PB BROWN, GV WOLFE - Journal of Eukaryotic Microbiology, 2006 - Blackwell Synergy ... Excess dye terminators were removed with CleanSEQ (Agencourt Biosciences) before sequencing on an ABI 310 genetic analyzer using 47-cm capillaries, yielding ...
PrP genotypes of atypical scrapie cases in Great Britain GC Saunders, S Cawthraw, SJ Mountjoy, J Hope, O ... - Journal of General Virology, 2006 - Soc General Microbiol ... min. Prior to loading on an ABI 3100 genetic analyser, the sequencing product was purified by using a CleanSEQ kit (Agencourt). ...
Preliminary demonstration of an allelic association of the IREB2 gene with Alzheimer's disease KD Coon, AM Siegel, SJ Yee, TL Dunckley, C Mueller ... - Journal of Alzheimer's Disease, 2006 - IOS Press ... Sequencing reac- tions were purified using CleanSEQ (Agencourt Bio- sciences Corp., Beverly, MA) to remove unincorpo- rated dye-terminators, and analyzed on ...
Epidermal Growth Factor Receptor Mutation Testing in the Care of Lung Cancer Patients LV Sequist, VA Joshi, PA Janne, DW Bell, P Fidias, ... - Clinical Cancer Research, 2006 - AACR ... Sequencing products are purified using CleanSEQ magnetic beads (Agencourt) and separated by capillary electrophoresis on an ABI 3730 DNA Analyzer (Applied ...
Characterization of the ?? T cell response to acute leukemia PF Meeh, M King, RL O'Brien, S Muga, P Buckhalts, ... - Cancer Immunology, Immunotherapy, 2006 - Springer ... thermal cycling blocks. Sequencing reac- tions were purified using Agencourt's CleanSEQ dye- terminator removal kit. Sample sheets ...
Time-Dependent Interaction Between Lopinavir/Ritonavir and Fexofenadine RPG van Heeswijk, M Bourbeau, P Campbell, I Seguin ... - The Journal of Clinical Pharmacology, 2006 - jcp.sagepub.com ... Removal of unincorporated fluorescent dyes was achieved by the use of CleanSEQ Magnetic Bead Purification (AgenCourt, Beverly, Mass). ...
A Polymerase Chain Reaction Screening Method For Rapid Detection Of Microsatellites In Bacterial Artificial Chromosomes MF Rodriguez, SA Gahr, CE Rexroad, Y Palti - Marine Biotechnology, 2006 - Springer ... s, 50-C for 20 s, and 60-C for 4 min and a final 5-min extension step at 60-C. Unincorporated dntps and primers were removed using the CleanSEQ magnetic bead...
No VSX1 Gene Mutations Associated with Keratoconus AJ Aldave, VS Yellore, AK Salem, GL Yoo, SA Rayner ... - Investigative Ophthalmology & Visual Science, 2006 - ARVO ... Unincorporated nucleotides were removed using the CleanSEQ reagent and a SPRI plate (Agencourt Bioscience Corp., Beverly, MA) according to the manufacturer's ...
Analysis of RNA splicing defects in PITX2 mutants supports a gene dosage model of Axenfeld-Rieger syndrome NL Maciolek, WLM Alward, JC Murray, EV Semina, MT ... - BMC Medical Genetics, 2006 - biomedcentral.com ... Extension products were purified using Agencourt CleanSEQ ® Reaction Clean up. PCR products were then sent to Agencourt, Inc to be sequenced. ...
Low-Cost, Simultaneous, Single-Sequence Genotyping Of The HLA-A, HLA-B And HLA-C Loci WGH Abbott, CF Tukuitonga, M Ofanoa, SR Munn, EJ ... - Tissue Antigens, 2006 - Blackwell Synergy ... The sequencing reaction products were purified by either ethanol precipitation for POP4 analysis or by magnetic beads (cleanSEQ, Agencourt Biosciences Corp ...
On The Use Of DNA Sequences For Determining The Species Limits Of A Polymorphic New Species In The Stink Bug Genus Halys (Heteroptera: Pentatomidae) From Pakistan N MEMON, R MEIER, A MANAN, KFYI SU - Systematic Entomology, 2006 - Blackwell Synergy ... 2 ?l of template, 1 ?l of Big Dye, 1.75 ?l of primer, 3 ?l of sequencing buffer and 2.25 ?l of H 2 O. The sequencing reactions were cleaned with CleanSEQ. ...
Human P2X7 Pore Function Predicts Allele Linkage Disequilibrium LC Denlinger, DB Coursin, K Schell, G Angelini, DN ... - Clinical Chemistry, 2006 - 171.66.123.124 ... Bidirectional sequencing was performed with the BigDye Kit (Applied Biosystems) and CleanSEQ magnetic beads (Agencourt Bioscience). ...
Evaluation of the TB-Biochip Oligonucleotide Microarray System for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis JC Caoili, A Mayorova, D Sikes, L Hickman, BB ... - Journal of Clinical Microbiology, 2006 - Am Soc Microbiol ... The CleanSEQ sequencing reaction clean-up system with SPRI paramagnetic beads (Agencourt, Beverly, MA) was utilized for removal of unincorporated dye terminator ...
Sequence analysis of the complete mitochondrial DNA in 10 commonly used inbred rat strain NE Schlick, MI Jensen-Seaman, K Orlebeke, AE ... - American Journal of Physiology- Cell Physiology, 2006 - Am Physiological Soc ... 5 sec, and 60°C for 4 min. Reactions were purified with the CleanSEQ dye-terminator removal system (Agencourt, Beverly, MA). DNA ...
Small-Molecule MDM2 Antagonists as a New Therapy Concept for Neuroblastoma T Van Maerken, F Speleman, J Vermeulen, I Lambertz ... - Cancer Research, 2006 - AACR ... Sequencing reactions were cleaned up with the CleanSEQ magnetic bead system (Agencourt, Beverly, MA), loaded onto an ABI3730 XL DNA sequencer (Applied ...
Mutation analysis of the seven in absentia homolog 1 (SIAH1) gene in Parkinson's disease T Franck, R Krueger, D Woitalla, T Müller, S ... - Journal of Neural Transmission, 2006 - Springer ... As 1 Q-Solution damages the dHPLC-column, pooled probes of the first exon were washed using CleanSEQ + (Agencourt) according to the ...
Basilar-type migraine Clinical, epidemiologic, and genetic features M Kirchmann, LL Thomsen, J Olesen - Neurology, 2006 - AAN Enterprises ... Cycle sequencing reactions were set up on Sciclone ALH 300, run on MJR Tetrad, and excess dye terminators were removed using CleanSEQ from Agencourt. ...
A Novel Gene (Cmya3) Induced in the Heart by Angiotensin II-Dependent but not Salt-Dependent Hypertension in Mice A Duka, F Schwartz, I Duka, C Johns, E Melista, I ... - American Journal of Hypertension, 2006 - Elsevier ... The sequencing reaction products were purified using the Agencourt CleanSEQ purification kit (Agencourt) and resolved by capillary electrophoresis using the ...
Phylogenetic relationships within Serpulidae (Sabellida, Annelida) inferred from molecular and morphological data EK Kupriyanova, T Macdonald, GW Rouse - Zoologica Scripta, 2006 - Blackwell Synergy ... Mastercycler. Sequenced products were purified using the magnetic method with CleanSEQ kit by Agencourt Biosciences Corporation. ...
Phylogeny And Biogeography Of Croton Alabamensis (Euphorbiaceae), A Rare Shrub From Texas And Alabama, Using DNA Sequence And AFLP Data BW VAN EE, N JELINSKI, PE BERRY, AL HIPP - Molecular Ecology, 2006 - Blackwell Synergy ... which two combinations were chosen: EcoRI + ACG/MseI + CCA and EcoRI + ACT/MseI + CTC (Table 2). Final PCR products were cleaned using CleanSEQ beads (Amersham ...
The CACNA1A And ATP1A2 Genes Are Not Involved In Dominantly Inherited Migraine With Aura M Kirchmann, LL Thomsen, J Olesen - Am J Med Genet B Neuropsychiatr Genet, 2006 - doi.wiley.com ... Cycle sequencing reactions were set up on Sciclone ALH 300, run on MJR Tetrad TM and excess dye terminators were removed using CleanSEQ TM from Agen- court. ...
Staminal Evolution in the Genus Salvia (Lamiaceae): Molecular Phylogenetic Evidence for Multiple Origins of the Staminal Lever J WALKER, KJ SYTSMA - Annals of Botany, 2006 - Annals Botany Co ... Sequenced products were precipitated in ethanol and sodium acetate to remove excess dye terminators or cleaned with the CleanSEQ Sequencing Reaction Clean-up ...
Phylogenetic analysis of mutualistic filamentous bacteria associated with fungus-growing ants MJ Cafaro, CR Currie - Can J Microbiol, 2005 - article.pubs.nrc-cnrc.gc.ca ... 16S-350R: 5?-CTGCTGCCTCCCGTAGGAGTCTG-3?, 16S-850R: 5?-CTT- TGAGTTTTAGCCTTGCGG-3?). Sequencing reactions were cleaned using CleanSEQ ® reaction Clean ...
Variability of CYP3A7 Expression in Human Fetal Liver JS Leeder, R Gaedigk, KA Marcucci, A Gaedigk, CA ... - Journal of Pharmacology and Experimental Therapeutics, 2005 - ASPET ... Unincorporated fluorescent dye-terminators were removed from the reactions by solid-phase magnetic CleanSEQ beads (Agencourt, Beverly, MA) according to the ...
Genetic Testing In Atypical HUS And The Role Of Membrane Cofactor Protein (MCP; CD46) And Factor I THJ Goodship, V Fremeaux-Bacchi, JP Atkinson - 2005 - Springer ... by dye terminator cycle sequencing using either exon-spe- cific primers or N13 primers, purified using magnetic microparticles (CleanSEQ, Agencourt Biosciences ...
Genomic Characterization Of non-O1, non-O139 Vibrio cholerae Reveals Genes For A Type III Secretion System M Dziejman, D Serruto, VC Tam, D Sturtevant, P ... - Proceedings of the National Academy of Sciences, 2005 - National Acad Sciences ... Products were purified using the CLEANSEQ system (Agencourt) on BIOMEK FX (Beckman Coulter) and applied to an ABI 3730 sequencer. ...
Methods And Compositions For Genetically Detecting Improved Milk Production Traits In Cattle H Khatib - 2005 - freepatentsonline.com ... degree.. Excess dye terminators were removed using CleanSEQ magnetic bead sequencing reaction clean up kit from Agencourt Biosciences. ...
Measurement Of In Situ Expression Of Proteorhodopsin Genes At The North Pacific Central Gyre Station Aloha Y Shi - 2005 - drum.umd.edu ... A novel approach was developed for the measurement of microbial gene expression in the oceans, and used to test the hypothesis that Proteorhodopsin (PR) gene expression...
Specificity Of The Mutualistic Association Between Actinomycete Bacteria And Two Sympatric Species Of Acromyrmex Leaf-Cutting Ants M Poulsen, M Cafaro, JJ Boomsma, CR Currie - Molecular Ecology, 2005 - Blackwell Synergy ... Sequencing reactions were performed using CEQ 2000 Dye Terminator Cycle Sequencing with Quick Start Kit (Beckman Coulter), cleaned using CleanSEQ TM reaction ...
Evolution and diversification of a sexually dimorphic luminescent system in ponyfishes (Teleostei: Leiognathidae), including diagnoses for two new genera JS Sparks, PV Dunlap, WL Smith - Cladistics, 2005 - Blackwell Synergy ... The sequencing reactions were cleaned and desalted using standard isopropanol-ethanol precipitation or using cleanSEQ (Agencourt Biosciences Corporation). ...
Evaluation Of 7sl Rna Gene Sequences For The Identification Of Leishmania Spp AM ZELAZNY, DP FEDORKO, LI LI, FA NEVA, SH FISCHER - The American Journal of Tropical Medicine and Hygiene, 2005 - ASTMH ... Sequencing products were purified with CleanSEQ Sequencing Reaction Clean-Up system (Agencourt, Beverly, MA) and analyzed using the 3100 Genetic Analyzer ...
Update of the molecular basis of familial hypercholesterolemia in the Netherlands SW Fouchier, JJ Kastelein, JC Defesche - Hum Mutat, 2005 - doi.wiley.com ... Sequence reactions were cleaned using CleanSEQ according to a protocol provided by the manufacture (Agencourt, Beverly, MA) and run on an automated DNA ...
Molecular phylogenetics and patterns of floral evolution in the Ericales. J Schoenenberger, AA Anderberg, KJ Sytsma - International Journal of Plant Sciences, 2005 - journals.uchicago.edu ... 2000) were used to sequence double-stranded DNA. Cycle-sequencing products were cleaned using CleanSEQ magnetic particles (Agencourt Biosience). ...
Catalysis of Protein Disulfide Bond Isomerization in a Homogeneous Substrate EA Kersteen, SR Barrows, RT Raines - Biochemistry, 2005 - biochem.wisc.edu ... 44, No. 36, 2005 12169 Page 3. BigDye kit from Applied Biosystems (Foster City, IA) and CleanSEQ magnetic beads from Agencourt Bioscience (Bev- erly, MA). ...
A Unique Corneal Dystrophy of Bowman's Layer and Stroma Associated with the Gly623Asp Mutation in the Transforming Growth Factor ?-Induced (TGFBI) Gene AJ Aldave, SA Rayner, JA King, JA Affeldt, VS ... - Ophthalmology, 2005 - Elsevier ... Unincorporated nucleotides were removed using the CleanSEQ reagent and an SPRI plate (Agencourt Bioscience Corp., Beverly, MA) following the manufacturer's ...
Linkage map construction in allotetraploid creeping bentgrass (Agrostis stolonifera L.) N Chakraborty, J Bae, S Warnke, T Chang, G Jung - TAG Theoretical and Applied Genetics, 2005 - Springer ... Reactions were then purified using CleanSEQ reagents (Agencourt, Beverly, Mass., USA), and then run on an ABI3700 sequencer at the University of Wisconsin ...
Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves T Nanjo, N Futamura, M Nishiguchi, T Igasaki, K ... - Plant and Cell Physiology, 2004 - Jpn Soc Plant Physiol ... The products of the sequencing reaction were purified by using a CleanSEQ Dye-Terminator Removal Kit (Agencourt Bioscience, Beverly, MA) and then loaded onto ...
Pattern of Sequence Variation Across 213 Environmental Response Genes RJ Livingston, A von Niederhausern, AG Jegga, DC ... - Genome Research, 2004 - Cold Spring Harbor Lab ... sequencing chemistry (ABI). Reactions were precipitated in ethanol with CleanSEQ magnetic beads (Agencourt). Packard Minitrack and ...
Near-Infrared Time-Resolved Fluorescence Lifetime Determinations In Poly(Methylmethacrylate) Microchip Electrophoresis Devices SD Llopis, W Stryjewski, SA Soper - Electrophoresis, 2004 - doi.wiley.com ... pro- cedure to remove excess salts, enzymes, dNTPs, and ddNTPs, a solid-phase reversible immobilization (SPRI) technique using CleanSEQ magnetic particles ...
Method For Isolating Nucleic Acids K McKernan, J Ziauddin - 2004 - freepatentsonline.com ... 2.5 minutes, 95 C for 5 seconds) [0095] 4 C until cleanup Purify sequencing reactions using either standard EtOH precipitation of Agencourt CleanSEQ kit (cat. ...
Identification of Mycobacterium Species by secA1 Sequences AM Zelazny, LB Calhoun, L Li, YR Shea, SH Fischer - Journal of Clinical Microbiology, 2004 - Am Soc Microbiol ... Sequencing products were purified with a CleanSEQ Sequencing Reaction Clean-Up system (Agencourt, Beverly, Mass.) and were analyzed with the 3100 Genetic ...
Mutations in PITX2 may contribute to cases of omphalocele and VATER-like syndromes LA Katz, RE Schultz, EV Semina, CP Torfs, KN Krahn ... - Am J Med Genet A, 2004 - doi.wiley.com ... 278 Katz et al. Page 3. 5 sec and 608C for 4 min. Extension products were purified using Agencourt CleanSEQ 1 Reaction Clean up. ...
Evidence For Landscape-Level, Pollen-Mediated Gene Flow From Genetically Modified Creeping Bentgrass With CP4 EPSPS As A Marker LS Watrud, EH Lee, A Fairbrother, C Burdick, JR ... - Proceedings of the National Academy of Sciences, 2004 - National Acad Sciences ... manufacturer (Applied Biosystems). Labeled fragments were purified with CleanSEQ kits (AgenCourt Bioscience, Beverly, MA). Sequence data ...
2-Methylcitrate-Dependent Activation Of The Propionate Catabolic Operon (Prpbcde) Of Salmonella Enterica By The Prpr Protein S Palacios, JC Escalante-Semerena - 2004 - Soc General Microbiol ... the manufacturer's instructions. Sequencing reactions were cleaned using CleanSEQ magnetic beads (Agencourt). Sequencing was performed ...
The acnD Genes of Shewenella oneidensis and Vibrio cholerae Encode a New Fe/S-Dependent 2-Methylcitrate Dehydratase Enzyme That Requires prpF Function in vivo TL Grimek, JC Escalante-Semerena - Journal of Bacteriology, 2004 - Am Soc Microbiol ... Reactions were purified by means of the CleanSEQ reaction clean-up protocol of Agencourt Bioscience Corpora- tion (Beverly, Mass.) and sequenced at the ...
Salvia (Lamiaceae) Is Not Monophyletic: Implications For The Systematics, Radiation, And Ecological Specializations Of Salvia And Tribe Mentheae JB Walker, KJ Sytsma, J Treutlein, M Wink - American Journal of Botany, 2004 - botany.wisc.edu ... Sequenced products were precipitated in ethanol and sodium acetate to remove excess dye ter- minators or cleaned with CleanSEQ Sequencing Reaction Clean-up ...
PAK Paradox: Paramecium Appears To Have More K+-Channel Genes than Humans WJ Haynes, KY Ling, Y Saimi, C Kung - Eukaryotic Cell, 2003 - Am Soc Microbiol ... The reaction products were purified by using CleanSEQ (Agencourt, Beverly, Mass.) and sequenced at the University of Wisconsin Biotechnology Center, Madison. ...
Genetic Heterogeneity in the rRNA Gene Locus of Trichophyton tonsurans A Gaedigk, R Gaedigk, SM Abdel-Rahman - Journal of Clinical Microbiology, 2003 - jcm.highwire.org ... The reactions were cycle sequenced as recommended, cleaned up with the CleanSEQ magnetic bead system (Agencourt, Beverly, Mass.), and analyzed on a MegaBACE ...
Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis W Hu, Y Wang, C Bowers, H Ma - Plant Molecular Biology, 2003 - Springer ... the ABI prism ® dGTP BigDye terminator v3.0 ready reaction cycle sequen- cing kit, and the extension products were purified with the CleanSEQ Reaction Clean ...
Phylogeny Of Eastern North American Coreopsis (Asteraceae-Coreopsideae): Insights From Nuclear And Plastid Sequences, And Comments On Character Evolution DJ Crawford, ME Mort - 1992 - Botanical Soc America ... al., 1997 ). Cycle sequencing reactions were purified using CleanSEQ (Agencourt Bioscience, Beverly, Massachusetts, USA). All contigs ...
Phylogenetics And Diversification Of Cotyledon (Crassulaceae) Inferred From Nuclear And Chloroplast DNA Sequence Data ME Mort, N Levsen, CP Randle, E Van Jaarsveld, A ... - American Journal of Botany, 1992 - Botanical Soc America ... al., 1990 ). Cycle sequencing reactions were purified using CleanSEQ (Agencourt Bioscience Corp., Beverly, Massachusetts, USA). All ...
Taxonomic Status of Acris Gryllus Paludicola: In Search of the Pink Frog FL Rose, TR Simpson, MRJ Forstner, DJ McHenry, J ... - Journal of Herpetology - bioone.org ... Products were cleaned with CleanSEQ® Dye Terminator Removal (Agencourt Bioscience Corporation) and analyzed on a CEQ TM 8800 Genetic Analysis System (Beckman ...
Contrasts in Cellulolytic Activities of Gut Microorganisms Between the Wood Borer, Saperda vestita (Coleoptera: Cerambycidae), and the Bark Beetles, Ips pini and Dendroctonus frontalis (Coleoptera: Curculionidae) I Delalibera, J Handelsman Jr, KF Raffa - Environmental Entomology - bioone.org ... ribosomal RNA gene. Samples were cleaned with CleanSEQ reaction clean-up (Agencourt Bioscience, Beverly, MA). Sequence determination ...
Phylogeographic Structure Of The Fire Ant Solenopsis Invicta In Its Native South American Range: Roles Of Natural Barriers And Habitat Connectivity ME Ahrens, KG Ross, DDW Shoemaker - Evolution - bioone.org ... Sequencing reactions were purified using Agencourt CleanSEQ magnetic beads and run on an ABI 3700 sequencer at the University of Wisconsin Biotechnology Center ...
Allelic And Haplotypic Diversity At The Major Histocompatibility Class II Within Domesticated Australian Atlantic Salmon (Salmo Salar L.) F Password - Blackwell Synergy ... Sequencing products were purified using the CleanSEQ Sequencing Reaction Clean-Up system (Agencourt Bioscience, Beverly, MA, USA). ...
No Pathogenic Mutations Identified in the COL8A2 Gene or Four Positional Candidate Genes in Patients with Posterior Polymorphous Corneal Dystrophy VS Yellore, SA Rayner, L Emmert-Buck, GC Tabin, I ... - Investigative Ophthalmology & Visual Science, 1321 - ARVO ... Unincorporated nucleotides were removed by using a dye terminator chemistry removal method (CleanSEQ reagent and an SPRI plate [solid phase reversible ...
The BARD1 Cys557Ser Variant and Breast Cancer Risk in Iceland SN Stacey, P Sulem, OT Johannsson, A Helgason, J ... - medicine.plosjournals.org ... were sequenced using an ABI PRISM Fluorescent Dye Terminator system (PerkinElmer; http://www.perkinelmer.com), repurified using CleanSEQ (Agencourt), and ...
Characterization of Gut-Associated Bacteria in Larvae and Adults of the Southern Pine Beetle, Dendroctonus frontalis Zimmermann A Vasanthakumar, I Delalibera, J Handelsman, KD ... - Environmental Entomology - bioone.org ... Sequenced products were purified either with Sephadex G-50 columns (Pharmacia Biotech, Piscataway, NJ) or CleanSEQ magnetic beads (Agencourt Bioscience). ...
Protocols for High-Volume DNA Barcode Analysis P By - barcoding.si.edu ... However, other options like SPRI (Solid Phase Reversible Immobilisation)-based dye terminator removal system eg AgenCourt CleanSEQ or ethanol precipitation ...
High-Throughput Species Identification: From DNA Isolation To Bioinformatics DE RICHARDSON, JD VANWYE, A EXUM, RK COWEN, DL ... - Blackwell Synergy ... Sequences were purified on the liquid-handling robot using the standard CleanSEQ kit protocol (Agencourt) and then run on a 3730 xl DNA Analyser (Applied ...
Grammar Synthesis as an Approach for Solving the Inverse Protein Folding Problem [DOC] G Synthesis, IPF Problem - cs.brandeis.edu ... Grammar Synthesis. as an Approach for Solving the. Inverse Protein Folding Problem. Kapil Mehra. Advisor: Jacques Cohen. Submitted for Senior Honors. ...
Casting the Percomorph Net Widely: The Importance of Broad Taxonomic Sampling in the Search for the Placement of Serranid and Percid Fishes WL Smith, MT Craig, JM Quattro - Copeia - bioone.org ... The sequencing reactions were cleaned and desalted using standard isopropanol-ethanol precipitation or using cleanSEQ (Agencourt Biosciences). ...
A High-Throughput Gene Knockout Procedure For Neurospora Reveals Functions For Multiple Transcription Factors HV Colot, G Park, GE Turner, C Ringelberg, CM Crew ... - Proceedings of the National Academy of Sciences, 2006 - National Acad Sciences ... with slight modifications: Zymolyase T-100 (Seikagaku America, Falmouth, MA) was used and, in place of the final precipitation step, CleanSEQ magnetic beads ...
The Tallysomycin Biosynthetic Gene Cluster From Streptoalloteichus Hindustanus E465-94 ATCC 31158 Unveiling New Insights Into The Biosynthesis Of The Bleomycin Family Of Antitumor Antibiotics M Tao, L Wang, E Wendt-Pienkowski, NP George, U ... - rsc.org ... The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the ...
Casting the Percomorph Net Widely: The Importance of Broad Taxonomic Sampling in the Search for the Placement of Serranid and Percid Fishes WL Smith, MT Craig, JM Quattro - Copeia - bioone.org ... The sequencing reactions were cleaned and desalted using standard isopropanol-ethanol precipitation or using cleanSEQ (Agencourt Biosciences). ...
The Tallysomycin Biosynthetic Gene Cluster From Streptoalloteichus Hindustanus E465-94 ATCC 31158 Unveiling New Insights Into The Biosynthesis Of The Bleomycin Family Of Antitumor Antibiotics M Tao, L Wang, E Wendt-Pienkowski, NP George, U ... - rsc.org ... The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the ...